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SOP for Operation and Cleaning of Laminar Bench

Objective
To lay down a procedure for operation and cleaning of Laminar bench.

Responsibility
Microbiologist/Executive.

Accountability
Executive

Procedure

Introduction 
  • A laminar flow clean air workstation is a device that provides a total sweep of a confined area because the entire body of air moves with uniform velocity along parallel lines, originating through a HEPA filter occupying one entire side of the confined area. Therefore, it bathes the entire area with very clean air, sweeping away contaminants. Laminar flow provides well-controlled work areas only if proper precautions are observed.


Working
  • Enter the sterility testing room/aseptic zone with proper gowning procedure.
  • Clean the LAF with IPA 70% solution daily before operation and after day's working.
  • Check the electric connection before starting the work.
  • Note the manometer reading. It should be zero if required set at 'zero'.
  • Switch ON the LAF unit and also the lights
  • Check the manometer reading (It should be between 10-15min.)
  • Switch ON the L.A.F. 1/2 hr. before sterility testing /aseptic operation.
  • The Pre-filter should be cleaned every 3 months

Annexure
Nil

Revision History
Nil


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