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SOP for Calibration of HPLC Column Oven

Objective
To provide a standard operating procedure for the calibration of the HPLC Column Oven.

Scope
This procedure is applicable to all HPLC Column Oven in QC Lab.

Responsibility
Doing : Technical Assistant/Executive
Checking : Executive /Manager

Accountability
Head of the Department

Procedure
Frequency: Once a year.


Calibration Procedure for HPLC Column Oven
  • Keep the sensor probe of reference calibrated digital thermometer inside the HPLC Column Oven and keep its position in about the center of the oven chamber.
  • Set the oven temp. at 25℃ .
  • Let the oven chamber to achieve a set temp. Allow it to stabilize it for at least 5 min.
  • After 5 minutes, observe the temp. in ref. thermometer.
  • Record the value of temp. shown on the instrument display and ref. the thermometer in Annexure–I.
  • Keep minimum five minutes intervals for the first and second reading.
  • Then set the oven temp. 40°C, 50°C, 60°C and 70°C each one by one and follow the procedure as mentioned in the above steps.

Abbreviations
°C: Degree centigrade.
HPLC: High-performance liquid Chromatography.

Revision History
Nil


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