Troubleshooting Problems with Column Backpressure

Possible Cause	Prevention/Solution
High Backpressure
Wrong pump setting	Check and correct setting
Normal for system	Increased backpressure normal if: Switched to a longer column Changed to smaller particles Changed to a smaller diameter Increased flow rate if no other changes are made
Pressure higher during the middle of the gradient	Normal
Temperature too low	Adjust column oven temperature
Column aging	Gradual increase in pressure normal over column lifetime
Blocked column frit	Reverse flush the column Use in-line filter Centrifuge or filter samples Use guard cartridges
Blocked in-line filter	Replace in-line filter frit Centrifuge or filter samples Pre-filter mobile phase
Blocked guard cartridge	Replace guard cartridge more frequently
System blockage	Systematically investigate system to find the blockage
Buffer precipitation	Reverse flush the column with water Review evaluation conditions
Low Backpressure
Leak in system	Locate leak and correct
Column temperature too high	Lower temperature
Flow too low	Increase flow rate

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