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SOP for Operation and Cleaning of HPLC Pump

To lay down a procedure for Operation and Cleaning of Operation and Cleaning of High-Performance Liquid Chromatography (HPLC) Pump (Make: Waters, 515 Pump).

The scope of this SOP is applicable for Operation and Cleaning of Operation and Cleaning of HPLC Pump (Make: Waters) in the Quality Control (QC) laboratory at PharmaSky Ltd.

QC Personnel: To operate and clean HPLC Pump.
QC Supervisor: To verify operation and cleaning of HPLC Pump.
QC Head: To ensure compliance to the procedure.


Procedure for Operation of HPLC pump
  • Ensure that all the cables and power cords of the pumps are connected properly.
  • Switch “ON” the HPLC Pump. The display will show, 515 Pump, 100 μl, Ver 1.0
  • After a few seconds, the display will show, Flow 1.000, Ready, 0.000 ml, – 25 psi
  • Press “Menu” till purge appears on the screen.
  • Turn the black valve anticlockwise to purge the mobile phase.
  • Press the EDIT/ ENTER key. The display will show messages ‘Start PURGE’ and ‘Press ENTER to confirm’.
  • Press the EDIT/ ENTER key to start purging.
  • The system will start the countdown from 5 minutes to zero. The display will show, PURGE5:00, RUN, 5.000 ml, -25 psi.
  • After purging close the valve by turning it clockwise.
  • Press “Menu” till ‘Flow’ appears on screen.
  • Press EDIT/ ENTER Key to change the flow rate. Press the Up “Δ” and Down “↓” arrow keys to set the required flow.
  • Press “Menu” key to register the set flow.
  • Record the operation carried out using the HPLC pump in respective format for recording.

Procedure for Cleaning of HPLC Pump
  • Clean the HPLC pump with a dry clean cloth.
  • Ensure proper cleaning of HPLC pump.
  • Record the cleaning of the HPLC pump in the respective format for recording.

During every operation and cleaning.


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