SOP for CHNS Analyzer (EuroEA 3000 series)

OBJECTIVE

This SOP describes the procedure for the operation of the CHNS analyzer (EuroEA 3000 series).

SCOPE

• CHNS Analyzer is a scientific instrument that can determine the elemental composition of samples such as organic and inorganic compounds.
• This instrument is used for qualitative and quantitative analysis of elements (CHNS) in pharmaceuticals.

RESPONSIBILITIES

• It is the responsibility of the QC Analyst to ensure adherence to this procedure.
• It is the responsibility of QC Manager to follow the instructions of this procedure.

DEFINITIONS

Bypass: Un-weighed standard samples are introduced to clean and condition the entire analytical circuit prior to sample analysis.

PRECAUTIONS

• Each time leak test has to be performed.
• You must not proceed with data acquisition until the leak test has passed.

PROCEDURE

Starting the Instrument

• Open the valves of the Helium and Oxygen gas cylinder and set the pressure to 4 bar (60 psi),
• Turn on the instrument, The on/off switch is located at the rear of the instrument.
• Turn on the computer and to Start the software, Double click the icon Callidus software.

Software Operation

• From the main menu click Operator login.
• Enter user name =EVR and Password = EA3000 and click on the OK button.
• Create a method for CHNS
• From the main menu select Instrument ….. Setting.
• In the method frame type, the instrument parameters as reported in the Analytical Protocol received with EA.
• Press the save as button to store the parameters in the methods library.
• Click on the send button to load the new parameters on the instrument. The front Furness and GC oven will start to heat up immediately.
• Wait for the instrument to condition, until the instrument status shows READY.

PEOPLE ALSO READ: SOP for Operation of Malvern Particle Size Analyzer

Performing the Leak test

• Click on the Instrument menu from the menu bar.
• Select the Leak test from the drop-down list
• Click on the START leak test Button.
• The result is displayed in the label reporting passed or failed.
• You must not proceed until the leak test has passed.

Setting Autorun Window

• Click on Analysis on the menu bar
• Select New autorun from the drop-down menu
• Fill in the sample table

Balance Interface: automatic transfer of weights

• Connect the balance cable to COM part #2 of your computer.
• Prep[are the sample table without entering weights for standards and samples.
• Click the balance button below the sample table.
• Click on the sample table the row of the position to which the weight must be transferred.
• Weigh the sample and wait for balance stability, then press the transfer button of the balance.
• The transferred weight appears in the sample table at the active cell.
• The process is repeated with each subsequent standard and sample.

Naming Autorun

• Click on the save button in the autorun frame
• Create a new autorun name window opens
• Click add type, Type a new name, and click OK.

Running an Experiment

• When all the settings have been made in the Autorun window, press Send to the instrument.
• When the monitor window bar shows READY press START.
• The acquisition window appears and the acquisition starts.

Viewing the acquired Chromatograms and Results

• Click the acquired samples button in the Acquisition window
• Click the desired sample Table line.
• The results window reports the % element.

Shutting down the instrument

• Click on Operator on the main menu
• Select Exit Callidus
• Than press OK
• Turn off instrument
• Close the gas cylinder valves.

ABBREVIATION

QC: Quality Control

ANNEXURE

Nil

REVISION HISTORY

Nil

PEOPLE ALSO READ: SOP for Calibration of Malvern Particle Size Analyzer