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General Test Procedure for Identification by UV

PURPOSE
To lay down the procedure for identification by UV

APPARATUS
UV Spectrophotometer

REAGENTS
NA

PROCEDURE
  • Dissolve a portion of the substance under examination in the designed medium (as per individual test procedure) to obtain a test solution having the concentration.
  • Similarly prepare a standard solution in the same manner.
  • Take UV absorption of sample and standard using 1 cm cells in the range 200 to 400 nm, unless specified in the individual test procedure.
  • The requirements are met if the ultraviolet absorption spectrum of the sample and standard exhibit maxima at the same wavelengths and absorptivities and or absorbance ratios are within the limit.

CALCULATION

REFERENCE

In-House

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