HOT POST

50/recent/ticker-posts

Header Ads Widget

SOP for Cleaning, Operation and Monitoring of Laminar Air Flow Unit

Objective:
To describe the procedure to be followed for cleaning, operation & monitoring of laminar airflow.

Scope:
Applicable to Laminar Airflow unit in Microbiology Laboratory of Quality Control Department.

Responsibility:
Lab Attendant: Cleaning the outer surface of LAF daily.
Microbiologist: To perform the analysis and clean the bench of LAF before and after completion of work.

Accountability:
Head - Quality Control.


Procedure:

Cleaning:
Daily: 
  • Clean the outer surface of the laminar airflow unit (LAF) with a cotton cloth soaked in 70% v/v IPA (0.45 m filtered) followed by wiping with a dry cotton cloth.
  • Before starting & after completion of any work, clean the bench of LAF with a cotton cloth soaked in 70% IPA (0.45 m filtered).


Operation:
  • Ensure the cleanliness of the laminar airflow bench.
  • Check the manometer scale before starting LAF, the scale must show the level as “0”.
  • Switch ON the main power supply. Switch ON the laminar airflow unit by operating the ON/OFF switch located at an upper panel of laminar airflow.
  • Start the laminar airflow approx. 15 minutes prior to use.
  • Ensure that the manometer scale of all LAF is more than 10 mm. If the manometer reading falls below 10mm, then inform the engineering department.
  • Note down the reading as per the SOP Titled “Monitoring of differential Pressure in Laminar Air Flow”.
  • After completion of work clean the LAF.
  • Switch OFF the laminar airflow & main power supply.

Monitoring:
  • Ensure the cleanliness of laminar airflow.
  • Switch ON the laminar airflow by operating the ON/OFF switch located at an upper panel of laminar airflow.

Settle plate Exposure:
Frequency: Twice a week.
  • Expose 2 SCD agar plates in LAF for 1 hour and then close the lid of the plate as per SOP Titled” Environmental monitoring of microbiology laboratory”.
  • Collect all exposed plates & Incubate the plates in an inverted position at 30-35°C for 2 days followed by 20°C – 25°C for 3 days to check for microbial contamination.
  • After incubation observe the plates and counts the number of colonies. Record the results as per SOP Titled “Environmental monitoring of microbiology laboratory”.
  • Validation for DOP test, Velocity, and Particle count is to be performed by an Approved outside party (Frequency: Every six months).
  • After the validation put the label.

Annexure
Nil

Revision History
Nil

PEOPLE ALSO READ: SOP For Bowie Dick Test

Post a Comment

0 Comments

close