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SOP for Growth Promotion Test

OBJECTIVE
To lay down a procedure to check the growth promotion properties of the procured media & also to describes the procedure to be followed to evidence each lot of prepared or autoclaved media support the growth of microorganisms when all the environmental conditions for growth is provided.

SCOPE
The SOP is applicable to the media used for microbiological analysis in microbiology laboratory.

RESPONSIBILITY
Microbiologist : To perform the procedure as per SOP. 
Head – Quality Control : To ensure the compliance of the SOP.


PROCEDURE
Liquid broth medium (Growth Promotion / Positive Control):
  • Prepare culture dilution as per Growth Promotion Test.
  • Inoculate 1 ml of culture to the sterile culture media and incubate at recommended temperature specified for the organism as per Table -1.
  • The media complies the growth promotion test if luxuriant growth (turbidity) is observed in the inoculated tubes of liquid media or distinct colony formation on the agar media within 48 hours of incubation.
  • If the observations are without any microbial growth discard the batch of medium and reject the entire lot of media.
  • Observe inoculated media & enter.

Acceptance criteria:
  • Micro-organism must show turbidity in the inoculated broth media and distinct colony formation on the solid agar media within 48 hours duration.
Table- 1 (Routinely used media)

 

Culture Medium

 

Microorganism

Growth Temperature (°C)

Incubation Period

(Hours)

 

Condition

Trypton Soya Broth or Soyabean Casein Digest Medium

Staphylococcus aureus Pseudomonas aeruginosa Bacillus subtilis

 

22.5 ± 2.5

 

24 - 72

 

Aerobic

Potato Dextrose Agar / Broth

Candida albicans

22.5 ± 2.5

24 - 48

Aerobic

Bismuth Sulphite Agar / Brilliant Green Agar/Xylose lysine deoxycholate agar

 

Salmonella abony

 

32.5 ± 2.5

 

24 - 48

 

Aerobic

Tetrathionate Brilliant Green bile broth/ Selenite broth

 

Salmonella abony

 

32.5 ± 2.5

 

24

 

Aerobic

Cetrimide Agar/

Pseudomonas Agar

Pseudomonas aeruginosa

 

32.5 ± 2.5

 

24 - 72

 

Aerobic

Baird-Parker Agar / Mannitol Salt Agar/ Vogal Jonson Agar

 

Staphylococcus aureus

 

32.5 ± 2.5

 

24 - 48

 

Aerobic

MacConkey Agar / Broth/EMB agar/Fluid lactose medium.

 

Escherichia coli ATCC

 

32.5 ± 2.5

 

24 - 48

 

Aerobic

 

 

Soyabean Casein Digest Agar

Bacillus subtilis, Pseudomonas

aeruginosa, Staphylococcus aureus, Aspergillus niger, Candida albicans

 

 

32.5± 2.5

 

 

24-72

 

 

Aerobic


  • Any other selective / differential media, if required for microbiological testing, growth promotion tests to be performed with the microorganism specified by the manufacturer, mentioned on the label of particular media container.

ANNEXURE
Nil

REVISION HISTORY
Nil

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