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SOP for Calibration of Atomic Absorption Spectrometer

Objective
To check the performance of the equipment for reliable and accurate results.

Scope
This procedure is applicable to check the performance of the atomic absorption spectrophotometer installed in the quality control department.

Responsibility
Doing: Technical Assistant
Checking: Executive /Manager

Accountability
Head of the Department

Procedure
Frequency: Once in a three month

Flame System Sensitivity Check
  • Measure 2.0 ppm Copper standard solution.
Acceptance Criteria: The average of seven absorbance readings should be NLT 0.280 Abs and % RSD should be NMT 1.

Linearity in Flame Mode
  • Perform Linearity with at least three calibration points for Zinc (213.9 nm), Copper (324.7 nm) and K (766.5 nm).
Acceptance Criteria: Regression should be NLT 0.99

GFAAS Sensitivity Check
  • Measure 20 µL injection of 20 ppb Copper standard solution
Acceptance Criteria: The average of seven absorbance readings should be NLT 0.06 Abs and % RSD should be NMT 5.
Refer to the below chart for the temperature parameter.


Linearity in GFAAS Mode
  • Perform Linearity with at least three calibration points for Lead (283.3 nm)
Acceptance Criteria: Regression should be NLT 0.99

VGA MODE (Hydride Generator)

Linearity:
  • Perform Linearity with at least three calibration points for Mercury.
Acceptance Criteria: Regression should be NLT 0.99.

Linearity in Flame Mode
  • Perform Linearity with at least three calibration points for Copper (324.7 nm)
Acceptance Criteria: Regression should be NLT 0.99

Linearity in GFAAS Mode
  • Perform Linearity with at least three calibration points for Lead (283.3 nm)
Acceptance Criteria: Regression should be NLT 0.99

VGA MODE (Hydride Generator)
  • Linearity: Perform Linearity with at least three calibration points for Mercury.
Acceptance Criteria: Regression should be NLT 0.99


Maintenance
  • Check the exhaust system using the smoke.
  • Check the acetylene cylinder pressure. Ensure that the acetylene is not drawn into the instrument.
  • Inspect the gas hoses for any damage.
  • Turn on the gas supplies to the instrument and check all hoses and connections for leaks.
  • Check water connections for GFAAS operation.
  • Check the burner for deposits. If any, soak with detergent and clean with distilled water.
  • At frequent intervals inspect the inside of the graphite shroud which is mounted at the centre cooling block.
  • Using a cotton bud or similar material, ensure that the bore and injection hole is free from loose carbon or sample residues.

Abbreviations
AAS: Atomic Absorption Spectrophotometer.
FAAS: Flame Atomic Absorption Spectrophotometer.
GFAAS: Graphite Furnace Atomic Absorption Spectrophotometer.
VGA: Vapour Generation Accessory.
Abs: Absorbance
nm: Nanometer
ppb: Parts per billion
ppm: Parts per million
RSD: Relative Standard Deviation
NLT: Not Less Than.
NMT: Not More Than
QC: Quality control
QA: Quality Assurance

Revision History
Nil

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