HPLC of Peptides and Proteins: Methods and Protocols

The Handbook of HPLC for Pharmaceutical Scientists is an in-depth handbook that gives practical information on the application of high-performance liquid chromatography (HPLC) methods for pharmaceutical analysis. This guide was written by a group of experienced pharmaceutical scientists and covers a wide range of subjects, from the principles of HPLC to advanced methods and applications.

The handbook's first part covers the fundamentals of HPLC, such as instruments, column selection, sample preparation, and mobile phase selection. This section includes covers chromatography fundamentals, such as the many forms of chromatography and the variables that influence chromatographic separation. It offers practical advice and recommendations on how to maximize chromatographic separation for various analytes, such as small molecules, peptides, and proteins.

The second section of the handbook focuses on the practical aspects of HPLC analysis in pharmaceutical science, including method development, validation, and troubleshooting. This section covers the importance of method validation and provides guidelines for selecting appropriate validation parameters, such as accuracy, precision, linearity, and sensitivity. It also discusses common issues that can arise during HPLC analysis, such as peak tailing, column bleeding, and contamination, and provides practical advice on how to troubleshoot these problems.

The handbook's third section covers advanced HPLC methods and applications in pharmaceutical research, including hyphenated techniques like LC-MS/MS and LC-QTOF, as well as recent advances in the area such as supercritical fluid chromatography (SFC) and two-dimensional chromatography. This section also contains case studies and examples of how HPLC may be utilized in a variety of pharmaceutical science applications, such as drug discovery, formulation development, stability testing, and bioanalysis.

Overall, the Handbook of HPLC for Pharmaceutical Scientists is an essential resource for pharmaceutical scientists working in the field of HPLC analysis. It provides a comprehensive overview of the principles, techniques, and applications of HPLC in pharmaceutical science, and provides practical guidance and tips that can be applied to a wide range of analytical challenges in the pharmaceutical industry.

Hands-on experts from academia and industry comprehensively describe how to successfully perform all the critical HPLC techniques needed for the analysis of peptides and proteins. The methods range from commonly used techniques to those for capillary to large-scale preparative isolation.

The authors have also presented a number of specific applications as case studies to illustrate the analytical approaches to a particular separation or assay challenge, with examples drawn from contemporary fields in biochemistry and biotechnology. Follow step-by-step instructions that ensure experimental success Develop your own separation and analytical protocols for peptide and protein analysis.