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Method of Analysis for Abamectin Content

Chromatographic Conditions:
Column 250 mm × 4.6 mm, C18, 5 µm 
Flow Rate 1 mL/minute 
Column Temperature : 30°C 
Detection Wavelength 245 nm 
Injection Volume 5 µL 
Mobile Phase: Acetonitrile : water (80:20)

Standard Preparation:
Weigh out accurately 0.06 g of Abamectin reference standard of known purity into a 50 ml
volumetric flask. Dissolve and make up the volume with the methanol. Shake well to homogenise.

Sample Preparation: 
Weigh out accurately equivalent to the sample 0.06 g of Abamectin into a 50 ml volumetric flask. Dissolve and make up the volume with the methanol. Shake well to homogenise.

Procedure: 
Inject standard preparation in five replicate & record the chromatogram. Inject sample preparation in duplicate, record the chromatogram. Retention Time of Abamectin 8.9min approx.
Calculate the percent of assay by using the following formula: 

Calculation:





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