How to take Spectrum in Shimadzu UV-1800 in UV Probe Software?

The Shimadzu UV-1800 is a spectrophotometer that is used to analyze the absorption and transmission of light in various substances. It is a versatile and powerful instrument that is widely used in research laboratories and quality control settings. One of the key features of the Shimadzu UV-1800 is its ability to measure the spectrum of a sample, which provides valuable information about its chemical composition and physical properties.

The spectrum in Shimadzu UV-1800 refers to the range of wavelengths of light that are absorbed or transmitted by a sample. The UV-1800 uses a monochromator to generate a narrow beam of light at a specific wavelength, which is then directed through the sample. The amount of light that is absorbed or transmitted by the sample is measured by a detector, and this information is used to create a spectrum.

The spectrum of a sample is a unique fingerprint that can be used to identify its chemical composition and physical properties. Different substances absorb and transmit light at different wavelengths, and this is reflected in their spectra. For example, a protein will have a different spectrum than a DNA molecule, and a red dye will have a different spectrum than a blue dye.

The Shimadzu UV-1800 is capable of measuring spectra in the ultraviolet (UV) and visible (VIS) regions of the electromagnetic spectrum. The UV region covers wavelengths from 190 to 400 nm, while the VIS region covers wavelengths from 400 to 800 nm. By measuring spectra in both regions, the UV-1800 can provide a comprehensive analysis of a sample's properties.

One of the key applications of spectrum analysis in the UV-1800 is in the field of pharmaceuticals. Many drugs and drug-like molecules have specific absorption and transmission properties that can be detected using UV-Vis spectrophotometry. By measuring the spectrum of a drug, researchers can determine its purity, concentration, and chemical identity. This is important for ensuring the safety and efficacy of pharmaceutical products.

Another application of spectrum analysis in the UV-1800 is in the field of environmental science. Many pollutants and toxins absorb light at specific wavelengths, and this can be used to identify and quantify their presence in the environment. For example, the UV-1800 can be used to measure the spectrum of a water sample to detect the presence of contaminants such as pesticides or heavy metals.

  • Ultraviolet-visible (UV-vis) spectroscopy is used to obtain the absorbance spectra of a compound in solution or as a solid. What is actually being observed spectroscopically is the absorbance of light energy or electromagnetic radiation, which excites electrons from the ground state to the first singlet excited state of the compound or material. 
  • The UV-vis region of energy for the electromagnetic spectrum covers 1.5 - 6.2 eV which relates to a wavelength range of 800 - 200 nm. The Beer-Lambert Law, Equation-1, is the principle behind absorbance spectroscopy. 
  • For a single wavelength, A is absorbance, ε is the molar absorptivity of the compound or molecule in solution, b is the path length of the cuvette or sample holder (usually 1 cm), and c is the concentration of the solution.
A = εbc

  • All of these instruments have a light source (usually a deuterium or tungsten lamp), a sample holder and a detector, but some have a filter for selecting one wavelength at a time. The single-beam instrument (Figure-1) has a filter or a monochromator between the source and the sample to analyze one wavelength at a time. 
  • The double beam instrument (Figure-2) has a single source and a monochromator and then there is a splitter and a series of mirrors to get the beam to a reference sample and the sample to be analyzed, this allows for more accurate readings. 
  • In contrast, the simultaneous instrument (Figure-3) does not have a monochromator between the sample and the source; instead, it has a diode array detector that allows the instrument to simultaneously detect the absorbance at all wavelengths. The simultaneous instrument is usually much faster and more efficient, but all of these types of spectrometers work well.


In conclusion, the spectrum in Shimadzu UV-1800 is a powerful tool for analyzing the properties of a wide range of substances. By measuring the absorption and transmission of light at different wavelengths, the UV-1800 can provide valuable information about the chemical composition and physical properties of a sample. This has important applications in fields such as pharmaceuticals and environmental science, and the UV-1800 is widely used in research laboratories and quality control settings around the world.

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