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SOP for Operation of Karl Fischer Apparatus


OBJECTIVE
To lay down the procedure for operation of Karl Fischer apparatus.

SCOPE
This SOP shall be applicable to Quality Control Dept.

RESPONSIBILITY
Quality Control Supervisor

ACCOUNTABILITY
Ass. Manager Quality Control

PROCEDURE
  • Take about 150 ml of Karl Fischer reagent. The glassware used should be well dried as otherwise, the reagent will become useless due to moisture.
  • Put the Karl Fischer reagent in the reservoir bottle provided at the back and place the stopper immediately after addition of Reagent.
  • Remove large rubber cork and add some methanol to the moisture beaker to such an extent that the metal wires of the sensors in the beaker arefully immersed. Replace the cork immediately.
  • Connect leads of the relay to the small three-pin socket on the titrator. Then connect the sensor wires to a socket provided on the instruments. Adjust the speed of the magnetic stirrers such that the wire ends of the sensor do not touch the stirred and at the same time sensors should not be exposed to air.
  • Put the dispensing burette on top of Karl Fischer Reagent bottle and close it properly.
  • Connect the titrator to the main supply. Veego Home screen will be displayed.
  • Rotate Stirrer knob provided on the right side of the instrument. Stirrer will be on.
  • Adjust the speed of the magnetic stirrer in such a way that the wired ends of the sensors do not touch the stirrer and at the same time sensors should not be exposed to air.
  • Press Start key. Instrument Starts calculating and neutralizing the water previously present in the methanol by adding Karl Fischer reagent. Once methanol is neutralized repeat the same procedure till KF reading for methanol comes to zero.
  • Weigh the sample for water content is to be determined. Press start key and add sample to methanol for water determination. KF reading will be displayed.
  • Press “Smpl” key and add the weight of sample taken for analysis. Press enter key and then “%age, PPM, mgH2O” key to display water content present in the sample.

ABBREVIATIONS
SOP: Standard Operating Procedure
QA: Quality Assurance
QC: Quality Control
Dept.: Department
KF: Karl Fischer

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