SOP for Sampling and Testing of Equipment wash water and Swab sampling

OBJECTIVE

To lay down the procedure for the sampling and testing of equipment wash water and swab sampling

SCOPE

This SOP is applicable to all incoming wash water and swab samples.

RESPONSIBILITY

Execution : Lab Attendant – QC department.

Checking : Chemist and above – QC department.

PROCEDURE

Take a clean glass bottle with a lid
Take a pretreated hydrophilic swab and immerse the swab part in 10 ml of purified water present in a clean glass tube and close the tube with the lid.
Take 10 ml of purified water into a clean stoppered glass test tube.
Take precautions while collecting the wash water and swab sampling
Wear hand gloves and nose masks during wash water collection.
Take a clean bottle and collect the wash water from a predefined location and label the bottle with the sample name and location
Take the pre prepared swab and swab at pre-defined location at an area of 10 x 10 cm² surface area of the equipment in a defined fashion. Care should be taken while taking swab; swab should not touch other than 10 x 10 cm² area.

2. Testing Procedure

Prepare the standard solution of the substance being examined as per respective test procedure.
Set the instruments at described wavelength with respect to sample being analyzed.
Perform the blank correction of the instruments by using water in 1 cm cell.
After the blank correction put the sample solutions being examined in the 1 cm cell and measure the Absorbance.
Similarly measure the standard solution absorbance.
Note down the sample and standard absorbance readings and calculate the contents in the sample solution in ppm

Sample absorbance
Calculation = ------------------------------------ x Concentration of standard sol. = ppm
Standard absorbance

REFERENCE(s)

Nil.

ABBREVIATION(s)

Nil

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