SOP for Calibration of Shimadzu IR Affinity-1S Fourier Transform Infrared Spectrophotometer

OBJECTIVE:

To lay down a procedure for the Calibration of Shimadzu IRAffinity-1S system using Lab Solutions software. 

SCOPE:

This is applicable for the calibration of Shimadzu IRAffinity-1S system using Lab Solutions software for IRAffinity-1S system. 

RESPONSIBILITY:

• QC Executives are responsible for carrying out the calibration as per this procedure. 
• QC Manager shall ensure that the procedure is followed. 

FREQUENCY:

Calibration shall be done every six months and after maintenance or replacement of spare parts. 

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PROCEDURE: 

• Ensure that the FTIR System is placed on a clean and dust free platform. 
• Ensure that the instrument has been connected to power supply. Ensure that the all communication cords between the units are connected properly. 
• Dry indicator checks: the check must be done before turning the instrument ON. There are two indicators – an orange lamp as Dry indicator and green lamp as Power indicator on the front. Dry indicator light shows the humidity in the interferometer of the FTIR even if it is off. Always confirm that the Dry indicator is on. 
• Silica gel check: this check must also be done before turning the instrument ON. 
• Power spectrum check: this is done by clicking [BKG Scan] icon to perform background scan measurements. A power spectrum is displayed after scanning. 
• Click the [Data] tab and then set scan parameters such as the number of scans and resolution. 
• Enter filename and sample information as “polystyrene film” and save in a specific folder as “Calibration of FTIR”. 
• Insert in the polystyrene film provided in the sample chamber cassette of the FTIR and start measurements. 
• A typical spectrum of polystyrene will appear. 
• Check the instrument for the following parameter and record the results in an Attachments 1.

           Wave no accuracy 

           Resolution 

ATTACHMENTS: 

Attachments 1: Calibration Report of Fourier Transform Infrared Spectrophotometer.

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ABBREVIATIONS:

QC – Quality Control 

QA – Quality Assurance 

FTIR – Fourier Transform Infrared spectrophotometer 

IR – Infrared 

BKG Scan – Background Scan 

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